Dmitry Yakovlev, Ph.D

Assistant Scientist, Biochemical Research Service Laboratory, The University of Kansas, Lawrence, Kansas, USA

• 1979 - Ph. D. in Bioorganic Chemistry, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR
• 1975 - M. S., Bioorganic Chemistry, Lomonosov Institute of Fine Chemical Technology, Moscow, USSR
• 1973 - B. S., Organic Chemistry, Lomonosov Institute of Fine Chemical Technology, Moscow, USSR

• 1996 - Present - Assistant Research Scientist, Biochemical Research Service Laboratory, University of Kansas, Lawrence, Kansas, USA
  Peptide synthesis and purification, protein modification and separation, organic synthesis, mass spectrometry of biomolecules, amino acids analysis, HPLC analysis and separations.
• 1994 - 1995 - Visiting Professor, Medical Research Council, Laboratory of Molecular Biology (head Sir Aaron Klug), Cambridge, England
  Design degenerate oligonucleotide probes and primers, and studies their application for gene isolation and genome sequencing.
• 1989 - 1990 - Visiting Scientist, Department of Biochemistry, University of Turku (head Timo Korpela), Turku, Finland
  Synthesis of new compounds for labeling of oligonucleotides and peptides, synthesis of biotin 4-aminooxybutylamide as a reagent for biotinylated nucleic acids and proteins, and isolations of labeled proteins from cells.
• 1987 - 1996 - Senior Research Scientist, Laboratory of Chemistry of Enzymatic Activity Regulators, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Moscow
  Synthesis of new derivatives of nucleic acid components for specific labeling nucleic acids and proteins, determine new chemical reactions for modifications of nucleic acids and peptides, peptide synthesis and sequencing.
• 1979 - 1986 - Research Scientist, Laboratory of Chemistry of Nucleic Acids, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow
  Determine action of laser UV radiation on bases, nucleosides, oligonucleotides and DNA; determine action O-methylhydroxylamine on transforming DNA, and use laser chemistry for investigation high structure polynucleotides and nucleoprotein complexes, investigation binding of ribosomal proteins with nucleic acids by translation.
• 1977 - 1979 - Junior Scientist, Laboratory of Chemistry of Nucleic Acids, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow
  Synthesis of new compounds for labeling and modification of nucleic acids.
• 1975 - 1979 - Pre-doctoral fellow in Laboratory of Chemistry of Nucleic Acids, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow
  Synthesis of new purine and pyrimidine nucleosides.

Supervised graduate students, taught courses in Bioorganic Chemistry, Department of General Medicine and Department of Pharmacy Sechenov Medical Academy, Moscow.

Synthetic organic chemistry; protein and nucleic acid chemistry; DNA and protein sequencing; peptide synthesis; chemical modification and labeling of nucleic acids and proteins; photochemistry of nucleic acids and their components; HPLC, MS, all types of separations of nucleic acids and proteins.

• 1996 - Grant Russian Ministry of Education and Science , principal investigator.
  Synthesis of new bicyclic analogs of 7-deazapurine and uracil and their applications to investigation of genome.
• 1995 - 1996 - Russian Foundation for Basic Research Grant No.95-03-08054, principal investigator.
  New reagents for chemical and photochemical modification of nucleic acids: Development of photo- and radio-protectors for nucleic acids on the basis of peptides and carbohydrates.
• 1994 -1995 - European Community INTAS Grant No.1010-CT93-0026 (with D. M. Brown, Medical Research Council, Laboratory of Molecular Biology, Cambridge, England)
  The design of degenerate oligonucleotide probes and primers and their application to gene isolation and genome sequencing.
• 1994 - Russian Foundation for Basic Research Grant No.94-03-08981, principal investigator.
  Photochemical modifications of pyrimidine bases of the nucleic acids.

M. Alterman, B. Kornilaev, T. Duzhak and D. Yakovlev. Quantitative Analysis of Cytochrome P450 Isozymes by Means of Unique Isozyme-Specific Tryptic Peptides: A Proteomic Approach. Drug Metab. Dispos. 2005, 33(9), 1399-1407

N. Galeva, D. Yakovlev, T. Duzhak, Y. Koen and M. Alterman. Direct Identification of Cytochrome P450 Isozymes by MALDI-TOF Based Proteomic Approach. Drug Metab. Dispos. 2003, 31(4), 351-355

T. Duzhak, N. Galeva, D. Yakovlev and M. Alterman. Cytochrome P450 Isozymes Proteomic Approach to the Profiling of Cytochrome P450 Isozymes. Drug Metab. Dispos. 2003, 35, 129

T. Duzhak, N. Galeva, D. Yakovlev and M. Alterman. Profiling of Cytochrome P450 Isozymes. 12th North American ISSX Meeting: Biotransformation and Disposition of Xenobiotics. Drug Metabolism Reviews 2003, 65

D. M. Williams, D. Y. Yakovlev and D. M. Brown. Synthesis of a Tricyclic Purine Related to N6-Hydroxyadenine. J. Chem. Soc. Perkin Trans I 1997, 8, 1171-1179

D. Yakovlev. The Protective Properties of Carnosine and beta-Cyclodextrin in Photoreactions of Nucleic Acid Bases. Coll. Czech. Chem. Comm. Spec. Issue 1996, 61, 142-144

D. Y. Yakovlev, S. G. Skuridin, A. R. Khomutov, Y. M. Yevdokimov and R. M. Khomutov. The Reduction of Thymine Residues in DNA by the Combined Action of UV Light and Hypophosphite. J. Photochem. Photobiol. B: Biol. 1995, 29 (2-3), 119-123

D. N. Nikogosyan, Y. A. Repeyev, D. Y. Yakovlev, V. I. Salyanov, S. G. Skuridin and Y. M. Yevdokimov. Photochemical Alterations in DNA Revealed by DNA-Based Liquid Crystals. Photochem. Photobiol. 1994, 59 (3), 269-276

A. R. Khomutov, D. Y. Yakovlev and R. M. Khomutov. New Reaction for Modification of Nucleic Acids and their Components. I. Modification of 2, 4-Dioxopyrimidines with Hypophosphite. Coll. Czech. Chem. Comm. Spec. Issue 1990, 55 (1), 69-72

D. Y. Yakovlev, A. R. Khomutov and R. M. Khomutov. New Reaction for Modification of Nucleic Acids and their Components. II. Photochemically Induced Selective Reduction of Uracil and Thymine. Coll. Czech. Chem. Comm. Spec. Issue 1990, 55 (1), 73-76

A. M. Kritsky, A. R. Khomutov, D. Y. Yakovlev, A. G. Gabibov, A. G. Rabinkov and R. M. Khomutov. Method for the Introduction of the Reactive Functional Groups via Cytidine Residues and/or 3'-Terminal of the Nucleic Acids. Nucl. Acids Res. Symp. Ser. 1987, 18, 89-92

E. I. Budowsky, O. I. Kovalsky, N. A. Simukova, V. V. Tsyshewsky, D. Y. Yakovlev and L. B. Rubin. Effects of High-Intensity UV Radiation on Thymidilyl-3', 5’-Thymidine. Lasers in Life Sciences 1986, 1 (2), 151-169

E. I. Budowsky, O. I. Kovalsky, D. Y. Yakovlev, N. A. Simukova and L. B. Rubin. Footprinting of DNA Secondary Structure by High-Intensity (Laser) Ultraviolet Irradiation. FEBS Lett. 1985, 188 (1), 155-158

Y. V. Morozov, F. A. Savin, V. O. Chekhov, E. I. Budowsky and D. Y. Yakovlev. Photochemistry of N-Methoxyadenosine and N-Hydroxycytidine and Its Methyl Derivatives. I. Spectroscopic and Quantum Chemical Investigation of Ionic and Tautomeric Forms: syn-anti Isomerization. J. Photochem. 1982, 20, 229-252

D. Y. Yakovlev, N. A. Simukova, E. I. Budowsky, V. O. Chekhov, F. A. Savin and Y. V. Morozov. Photochemistry of N-Methoxyadenosine and N-Hydroxycytidine and Its Methyl Derivatives. II. Photoinduced Rupture of the N-O Bond. J. Photochem. 1982, 20, 253-268

E. I. Budowsky, D. N. Nikogosyan, A. A. Oraevsky, N. A. Simukova and D. Y. Yakovlev. Direct and Indirect Action on Thymine in Aqueous Solution by Powerful Laser Ultraviolet Radiation. Photobiochem. Photobiophys. 1982, 4, 233-239

A. V. Borodavkin, V. O. Chekhov, Y. S. Dolin, Y. V. Morozov, F. A. Savin, E. I. Budowsky and D. Y. Yakovlev. Absorption UV Spectroscopy and Electronic Structure of Ionic and Tautomeric Forms of Hydroxy and Methoxy Derivatives of Cytosine and Adenine, and Some 5-Substituted Analogs of Pyrimidines. Int. J. Quant. Chem. 1980, 17 (4), 803-811

N. A. Simukova, D. Y. Yakovlev and E. I. Budowsky. Mechanism of the Mutagenic Action of Hydroxylamine. IX. The UV-Induced Cleavage of the N-O Bond in N-Hydroxy- and N-Methoxycytidine and N-Methoxyadenosine. Nucl. Acids Res. 1975, 2 (12), 2269-2278

M. Alterman, D. Yakovlev, T. Duzhak and B. Kornilaev. Qualitative and Quantitative MALDI- TOF-Based Proteomic Approach to the Profiling of Cytochrome P450 Isozymes. Keystone Symposia: Mass Spectrometry in Systems Biology, Santa Fe, New Mexico, USA, 2004

T. Duzhak, D. Yakovlev, B. Kornilaev and M. Alterman. Proteomic Approach to the Profiling of Cytochrome P450 Isozymes. ABRF Meeting: Integrated Technologies in Proteomics and Genomics, Portland, Oregon, USA, 2004

M. Alterman, N. Galeva, T. Duzhak and D. Yakovlev. Proteomics Approach to the Profiling of Cytochrome P450 Isozymes. ASBMB Special Symposium: Proteomic Solutions in Cellular and Developmental Biology and Medicine, Kansas City, USA, 2003

T. Duzhak, N. Galeva, D. Yakovlev and M. Alterman. Cytochrome P450 Isozymes Proteomic Approach to the Profiling of Cytochrome P450 Isozymes.12th North American ISSX Meeting: Biotransformation and Disposition of Xenobiotics, Providence, Rhode Island, USA, 2003

D. Y. Yakovlev. The Protective Properties of Carnosine and beta-Cyclodextrin in Photoreactions of Nucleic Acid Bases. 10th Symposium on the Chemistry of Nucleic Acid Components, Trest Castle, Czech Republic, 1996, Abstracts, S142

D. Y. Yakovlev. New Protective Properties of beta-Cyclodextrin in Photoreactions. 7th International Symposium on Cyclodextrins, Tokyo, Japan, 1994, Vol.7/Suppl., p. 89

D. Yakovlev, S. Skuridin, A. Khomutov, Y. Yevdokimov and R. Khomutov. Selective Reduction of Thymine by Combined Action of UV-Light and Hypophosphite. 22nd Meeting of the Federation of European Biochemical Societies, Stockholm, Sweden, 1993, Proceeding, p. 127

D. Yakovlev, S. Skuridin, A. Khomutov, Y. Yevdokimov and R. Khomutov. Selective Reduction of Thymine by Combined Action of UV-Light and Hypophosphite. 7th FECHEM Conference on Heterocycles in Bio-Organic Chemistry, Santiago de Compostela, Spain, 1993, Proceeding of Posters, PO-073

D. N. Nikogosyan, Y. A. Repeyev, D. Y. Yakovlev, V. I. Salyanov, S. G. Skuridin and Y. M. Yevdokimov. Photochemical Alterations in DNA Revealed by DNA-Based Liquid Crystals. 16th International Conference on Photochemistry, Vancouver, Canada, 1993, Abstracts, p. 499

D. N. Nikogosyan, Y. A. Repeyev, D. Y. Yakovlev, V. I. Salyanov, S. G. Skuridin and Y. M. Yevdokimov. Photolesions in DNA Revealed by DNA-Based Liquid Crystals. 5th Congress of the European Society for Photobiology, Marburg, Germany, 1993, Abstracts, p. 248

A. M. Kritsky, A. R. Khomutov, A. M. Shuster, D. Y. Yakovlev and R. M. Khomutov. New Method for Administration of the Reactive Functional Groups into the Nucleic Acids. 14th International Congress of Biochemistry, Prague, Czechoslovakia, 1988, Abstracts, v. 5, p. 81

E. I. Budowsky, D. Y. Yakovlev, N. A. Simukova, O. I. Kovalsky, M. S. Aksenteva, L. L. Orekhova, T. N. Menshonkova and L. B. Rubin. The Action of High Intensity Ultraviolet Irradiation on Nucleic Acids, Their Components and Nucleoproteins. 16th Meeting of the Federation of European Biochemical Societies, Moscow, USSR, 1984, Proceeding, p.405

D. Y. Yakovlev, N. A. Simukova, E. I. Budowsky, V. O. Chekhov, F. A. Savin and Y. V. Morozov. Photochemical Transformation of N-Hydroxy and N-Methoxy Derivatives of Cytidine and Adenosine. 7th International Congress of Photobiology, Strasburg, France, 1980, Abstracts, p. 235

A. V. Borodavkin, E. I. Budowsky, Y. S. Dolin, Y. V. Morozov, F. A. Savin, V. O. Chekhov and D. Y. Yakovlev. Electronic Structure and UV Spectroscopy Different Ionic and Tautomeric Forms of Hydroxy and Methoxy Derivatives of Cytosine and Adenine, and of Some 5-Substituted Derivatives of Uracil and Cytosine. International Conference on Quantum Chemistry, Biology and Pharmacology, Kiev, USSR, 1978, Abstracts of Posters, v. 3, p. 17

A. Khomutov, D. Yakovlev, R. Khomutov, T. Korpela. Derivatives of Cyclodextrins. US Patent No. 6,998,479 (2006)

A. Khomutov, D. Yakovlev, R. Khomutov, T. Korpela. Novel Derivatives of Cyclodextrins. WO Patent No. 045032 (1999)

A. R. Khomutov, A. G. Rabinkov, E. I. Vikhreva, D. Y. Yakovlev, A. M. Shuster, T. Korpela, A.M. Kritsky, A. G. Gabibov and R. M. Khomutov. 4-Aminooxybutylamide Biotin Hydrochloride as Reagent for Biotinylated Nucleic Acids and Proteins. Inventor's Certificate SU 1591458, 1990 (Russia)